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. 2022 Jan 20;3:795644. doi: 10.3389/fgeed.2021.795644

FIGURE 4.

FIGURE 4

Targeting the 5’ versus the 3’ end of DMR6-1 and effect of multiplexing. 6 gRNAs were designed to target the 5’ (g43, g44, and g45) (A) and 3’ end (g46, g47, and g48) (B) of DMR6-1, respectively. The gRNAs were tested individually and in combination. Theoretical designates the sum of the individual two gRNAs editing’s (2 biological replicates were performed with std errors indicated). gRNAs and IDAA primers used: g43, g44, and DMR6 Forward primer 1 + DMR6 Reverse primer 1; g45 and DMR6 Forward primer 2 + DMR6 Reverse primer 2; g46, g47 & g48 and DMR6 Forward primer 3 & DMR6 Reverse primer 3 (see Material and Methods).