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Effect of editing when simultaneously targeting (multiplexing) the 3’ end of GWD1 and DMR6-1, residing on different chromosomes. Single RNPs/gRNAs targeting the 3’ end of GWD1 (chromosome 5) and DMR6-1 (chromosome 3) were selected and employed individually or in combination. IDAA primer pairs used: GWD Forward primer 1, GWD Reverse primer 1 + DMR6 Forward primer 3 + DMR6 Reverse primer 3 (see Material and Methods).
