Figure 1.

Dynamic changes in γδT cells in the spleen of P. yoelii NSM infected mice. Fourteen days after Plasmodium infection, lymphocytes from the spleen, MLN, lung, liver, and PBMC of C57BL/6 mice were isolated. Different fluorescence-labelled mAbs against mouse CD3 and γδTCR were used to detect the content of γδT cells by flow cytometry. (A) All the doublet cells, dead cells, and nonlymphoid cells were excluded from flow cytometry data. (B, C) Dynamic changes in γδT cells in the spleens of C57BL/6 mice infected with Plasmodium were determined by FCM from day 4 to day 28, simultaneously setting up naive control. (D) Averages of γδT cells in infected mice in different organs were calculated after FCM analysis. Cell numbers of γδT cells from different organs in infected mice, compared with naive groups. Representative results of three independent results are shown. The average of three independent experiments with 3-5 mice per group was shown and repeated three times with similar results. The error bars are SD, *P < 0.05, **P < 0.01.