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. 2022 Jan 20;11:788546. doi: 10.3389/fcimb.2021.788546

Figure 2.

Figure 2

Plasmodium infection induced splenic γδT cell activation and differentiation. (A, B) Splenocytes were separated from naive and infected mice (14 days post infection) and then stained with monoclonal antibodies against mouse CD3, γδTCR, Vγ2, CD4, CD8, CD62 L, CD25, CD69, CD44, CD16/32, CD40 L, CD80, PD-1, PD-L1, PD-L2, ICOS, CXCR3 and CX3CR1 after cell surface staining. (A) A representative result of three independent experiments is shown. (B) The average expression of different surface molecules on γδT cells was calculated by FCM data. (C, D) Isolated splenocytes were stimulated by PMA and ionomycin, and the expression levels of cytokines (IFN-γ, IL-4, IL-5, IL-10, IL-2, and IL-17) were detected in CD3+ γδTCR+ cells after intracellular staining. (C) A representative of three independent experiments is shown. (D) The average expression levels of different cytokines on γδT cells were calculated by FCM data. The average of three independent experiments with 3-5 mice per group was shown and repeated three times with similar results. The error bars are SD, *P < 0.05, **P < 0.01. ns, no significance.