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. 2022 Jan 20;9:798609. doi: 10.3389/fped.2021.798609

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Copyright © 2022 Kontopodi, Boeren, Stahl, van Goudoever, van Elburg and Hettinga.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic workflow indicating the experimental approach used. HTST, FH, T99, T74, HoP and HoP_r stand for high-temperature short-time, flash heating, high-temperature short-time with a thermomixer preheated at 99°C, high-temperature short-time with a thermomixer preheated at 74°C, holder pasteurization and holder pasteurization with rapid heating up-times, respectively. For each heating method, DHM treatment was done in biological duplicate. Each sample was then aliquoted for the subsequent analyses, which were performed in technical duplicate.