Figure 1.

The novel expansion method, tumor-infiltrating lymphocytes (TIL) 3.0, enriches for CD3⁺ and CD8⁺ TIL while preserving T-cell diversity. (A) Schematic depicting TIL expansion from a single lung tumor resection. The minced tumor fragments are put in culture to propagate TIL with either the TIL 1.0 or TIL 3.0 method. (B) Comparison of the total TIL number expanded using the traditional culture method, TIL 1.0 (red) and the novel culture method, TIL 3.0 (blue) (paired, n=16). (C) The percentage of CD3⁺CD8⁺ TIL (left panel) and the percentage of CD3⁺CD4⁺ TIL (right panel) (paired, n=10) in successful TIL cultures for both expansion methods. (D) Time of culture with median of total TIL number expanded and days in culture (paired, n=16). The graph is divided in quadrants according to the median of TIL expanded versus number of days. (E) Percentage of patients in each quadrant for TIL 1.0 and TIL 3.0 from part D. (F) Success rate of growth for each TIL expansion method (paired, n=16). Comparison of T-cell receptor (TCR) richness (G) and clonality (H) of expanded TIL using TIL 1.0 vs TIL 3.0 (paired, n=9). Statistical analysis was performed by paired t-test (B) and (C), a Χ² test was performed in (E) and a sign-rank test was performed in (G) and (H).