Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Feb 2;10(2):e003082. doi: 10.1136/jitc-2021-003082

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2022. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Characterization of the pre-rapid expansion protocol (pre-REP) tumor-infiltrating lymphocyte (TIL) products reveals that TIL 3.0 expanded TIL are less differentiated than their post-REP counterpart. (A) Total fold expansion (left axis) of final TIL 1.0 product (red) and TIL 3.0 (blue) (paired, n=6). Gray circles indicate viability (right axis) above each TIL culture. (B) The percentage of CD3⁺CD8⁺ TIL (left panel) and the percentage of CD3⁺CD4⁺ TIL (right panel) (paired, n=6) in the final TIL product. (C) Assessment of the surface expression of the differentiation/activation CD27, CD28, programmed death-1 (PD-1) and LAG3 on pre-REP (solid square) and post-REP (open square) CD8⁺ TIL (in %) generated from TIL 3.0 culture (paired, n=6). (D) Comparison of proportion of the four distinct E_M population subsets of pre-REP and post-REP CD8⁺ TIL generated from TIL 3.0 culture (paired, n=6). (E) Analysis of TIL functionality measured by intracellular interferon (IFN)-γ, tumor necrosis factor (TNF)-α, co-expression and CD107a on fresh post-REP CD8⁺ TIL (in %) on phorbol myristate acetate (PMA)/ionomycin activation (TIL 1.0 vs TIL 3.0, paired, n=3). Statistical analysis was performed by paired t-test on (B), (C), (D) and (E).