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. 2021 Dec 20;23(2):e53902. doi: 10.15252/embr.202153902

Figure EV1. A DSB in the promoter of ABCB1 causes gene upregulation and Taxol‐resistance.

Figure EV1

  • A, B
    Example replicate of crystal violet staining of Colony Formation Assays with 8 nM of Taxol for RPE‐1 WT cells quantified in Fig 1A (A) and HMEC‐1 (parental) and cells infected with Lenti‐guides targeting the ABCB1 gene (sgRNAs #1 to #15) (B).
  • C
    Flow cytometry plot of RPE‐1 cells infected with BFP‐dCas9 construct. BFP positive cells (in red) were sorted and grown to perform sgRNA infections and CFA under Taxol selection. X‐axis shows GFP expression (autofluorescence).
  • D
    Example replicate of crystal violet staining of Colony Formation Assays with 8 nM of Taxol for RPE‐1 Cas9 and RPE‐1 dCas9 cells infected with the indicated sgRNAs targeting ABCB1.
  • E
    Quantification of D, error bars show the SD of three biological replicates (n = 3).