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. 2021 Dec 2;23(2):e53658. doi: 10.15252/embr.202153658

Figure 2. ACE2 levels increase upon telomere shortening in human cells and in lungs of G3 Terc−/− mice.

Figure 2

  1. RT‐qPCR detection of ACE2 mRNA expression levels in early passages (PD 34–37) and late passages (PD 61‐64) human normal fibroblasts (BJ) (n = 3–4 replicates per group).
  2. ddPCR detection of ACE2 mRNA expression levels in early passages (PD 22.37) and late passages (PD 140) human bronchial epithelial cells (HBEC) (n = 6 replicates per group).
  3. Representative images and quantitative analyses of ACE2 immunohistochemical staining in lungs of age‐matched wild‐type and G3 Terc−/− mice (n = 3–4 mice per group). Scale bar, 200 µm.
  4. Double‐marker immunofluorescence and quantitative analyses of ACE2 intensity level in pro‐SP‐C‐positive type II pneumocytic and CD31‐positive endothelia in lungs of age‐matched wild‐type and G3 Terc−/− mice (n = 3–4 mice per group). Scale bar, 100 µm. a.u. = arbitrary units.

Data information: In (A–D), data are presented as mean ± SEM. *P < 0.05, ***P < 0.001, ****P < 0.0001. Two‐sided unpaired Student's t‐test.