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Restriction analysis by BstI enzyme for PCR product of c.35delG mutation in GJB2 gene in 3% agarose gel electrophoresis. Lane M: DNA Ladder 50bp, Lane 1, 3, and 6 are heterozygous genotype (89+69 bp), and Lane 2, 4, 5,7, 8, and 9 homozygous genotypes (89 bp)of mutant alleles.
