Table 2.
Mutations and parameters for PCR-RFLP.
| Primer sequence | Mutation | PCR Product (bp) | Restriction Enzyme | Allele size (bp) | Ref. |
|---|---|---|---|---|---|
|
F:TCTTTTCCAGAGCAAACCGC
R:GCTGGTGGAGTGTTTGTTCACA |
c.35delG | 89 | BstI | Wild: 69+20 Hetero: 89+69 Homo: 89 |
[19] |
|
F: TGTGTGCATTCGTCTTTTCCAG
R:GGTTGCCTCATCCCTCTCAT |
c.235delC | 722 | ApaI | Wild: 451+277 Hetero: 722+451+277 Homo: 722 |
[28] |
|
F: GATTGGGGCACGCTGCA
R: CCCTTGATGAACTTCCTCTTCT |
c.167delT | 322 | MwoI | Wild: 322 Hetero: 322+161 Homo: 161 |
[2] |
bp – base pair; F – Foreword; R – Reverse; Ref – Reference.