FIG. 1.

Multiple aligment and clustering of the 23S-5S intergenic spacer region of B. burgdorferi sensu lato and reverse line blot result using the amplified spacer sequences. Multiple alignment and clustering were performed with the complete 174- to 182-bp spacer region and are only partially displayed. The coordinates of the B. burgdorferi sensu stricto spacer sequence are indicated above the sequences, and the probe regions are denoted by grey boxes. Reverse line blot probes are indicated as follows: VS, B. valaisiana; RS, B. afzelii like; AF, B. afzelii; GA, B. garinii; SS, B. burgdorferi sensu stricto; SL, B. burgdorferi sensu lato.