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The 2 K Q-band absorption EPR with the spectra of in vivo (black) and purified (red) Mc. sp. str. Rockwell pMMO overlaid. At this frequency, the +3/2 63Cu hyperfine of the CuC site is separated from the CuB site, as also shown by individual simulations of the two sites (inset). The asterisk marks a radical that is present in whole-cell samples. Conditions: ~34.8 MHz, 1 G modulation, 1 μW MW power, 128 ms time constant, 20 G/s sweep rate.
