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SDS-PAGE analysis of the cleavage test (n = 4). Abbreviation: L: protein ladder; PC: positive control; and 0–7: the target protein with 0, 2.5 × 10–6, 2.5 × 10–5, 2.5 × 10–4, 2.5 × 10–3, 2.5 × 10–2, 2.5 × 10–1, and 2.5 U/mL thrombin, respectively.
