Fig. 1.

Anti-tumor effect of CA IX-specific mouse monoclonal antibodies in vivo. A, B Inhibition of tumor growth by antibodies IV/18 and VII/20 on B16-CA IX cell xenografts stably transfected with human CA IX in C57BL/6J mice model. C, D Inhibition of lung metastasis formation of hypoxia pre-incubated HT1080-RFP cells treated by CA IX-specific antibody IV/18 in tail vein colonization assay. Data in the graph represent the mean ± standard deviation values. Statistical significance of the differences between antibody-treated and non-treated animals was assessed using Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001), n = 6