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. Author manuscript; available in PMC: 2022 Feb 3.
Published in final edited form as: Anal Chem. 2008 Mar 7;80(8):2932–2938. doi: 10.1021/ac7023969

Figure 1.

Figure 1.

(a) HPLC trace for the separation of the interstrand cross-link reaction mixture. The peaks at 38 (SM) and 49 min (ICL) correspond to the unreacted ODN d(CGCGCTAGCGCG) and the duplex ODN bearing an 8-MOP ICL, respectively. (b) Negative-ion ESI-MSofthenucleaseP1digestionproductsofd(CGCGCTAGCGCG). The ions of m/z 306, 321, 330, and 346 correspond to the [M – H]− ions of 2′-deoxycytidine 5′-phosphate (pdC), thymidine 5′-phosphate (pdT), 2′-deoxyadenosine 5′-phosphate (pdA), and 2′-deoxyguanosine 5′-phosphate (pdG), respectively. (c) Negative-ion ESI-MS of the nuclease P1 digestion products of self-complementary d(CGCGCTAGCGCG) cross-linked with 8-MOP. The ions of m/z 306 and 346 are the deprotonated ions of pdC and pdG, respectively. The origin for the ions of m/z 404 and 444 is not clear.