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. 2022 Jan 18;23(3):226. doi: 10.3892/etm.2022.11150

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Copyright: © Zhang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Protein expression levels of HO-1 regulator proteins in H9c2 cells using western blot method. (A) Western blotting analysis and quantitative analysis of (B) Keap1, (C) Nrf2, (D) HO-1, (E) IKK and (F) NF-κB in H9c2 cells. Data are presented as the mean ± SD. Each experiment was repeated three times independently. ^*P<0.05 vs. NC group. ^#P<0.05 vs. OT group. DHE, dehydrocostus lactone; TXNIP, thioredoxin-interacting protein; NC, negative control; OT, DHE treatment group; OI, DHE treatment combined with TXNIP inhibition group; OH, DHE treatment combined with TXNIP overexpression group; Nrf2, nuclear factor erythroid 2-related factor 2; HO-1, heme oxygenase-1; Keap1, Kelch-like ECH-associated protein 1; MW, molecular weight.