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. 2022 Jan 11;56(3):1675–1687. doi: 10.1021/acs.est.1c05410

Table 2. Biotic (B) and Spatial (S) Variables Pertinent to Testing the Association of PFAS Concentrations in Otters to Anthropogenic Sources.

variable detail data source GLMs variable included in
B otter length total length, nose to tip of tail (mm). Included to control for differences in concentration as a result of body size. Correlated with sex due to sexual dimorphism measured at post-mortem examination by Cardiff University Otter Project (CUOP) all
B otter sex male (n = 27) or female (n = 23). Differences between male and female may include offloading of pollutants to offspring for females determined at post-mortem examination by CUOP none, due to collinearity with length
B otter body condition scaled mass index (SMI) estimate of body condition (using Peig and Green34). Test for the potential impact of PFASs on body condition or vice versa calculated from length and weight measurements taken during post-mortem examination by CUOP all
S percentage landfill area percentage of land in 10 km buffer of otter that has historic or currently used landfill sites. Source of PFASs via the disposal of consumer products Natural Resources Wales47,48 and Environment Agency49,50 all
S percentage urban area percentage of urban and suburban land in the 10 km radius of otter. Source of PFASs via the use of consumer products, and the use of PFAS-based firefighting foam, including at airports U.K. Centre for Ecology and Hydrology—Land Cover Map 200751 none, due to collinearity with proportion landfill area
S mean wastewater treatment works load mean load entering WWTW, measured in population equivalent (PE), within a 10 km radius around each otter. Source of PFASs via the disposal of industrial and domestic wastewater European Environment Agency52 all
S percentage pastoral land percentage of improved grassland within a 10 km radius around each otter. Potential proxy for the application of sewage sludge on pastoral land U.K. Centre for Ecology and Hydrology—Land Cover Map 200751 all
S percentage arable land percentage of arable land within the 10 km radius around each otter. Potential proxy for the application of sewage sludge on arable land U.K. Centre for Ecology and Hydrology—Land Cover Map 200751 all
S rainfall mean rainfall (mm) within the 10 km radius around each otter from years 2006–2009. Source of PFASs via atmospheric deposition or dilution within river Met Office53 none, due to collinearity with proportion arable land
S distance to factory producing PTFE linear distance from each otter to a factory using PFOA at a time of sampling (2007–09) in PTFE manufacture (AGC Chemicals Europe Ltd, Lancashire) calculated using ArcGIS join tool to factory location54 PFOA only
S PFOS discharge to water binary variable, discharge reported, or no discharge reported within a 10 km radius around each otter pollution inventories for Wales and England55,56 PFOS only