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RT-qPCR performance characteristics and assay limit of detection (ALOD).
| Assay | Performance characteristics | ||||
|---|---|---|---|---|---|
| Efficiency (E) (%) | Linearity (R2) | Slope | Y-intercept | ALOD for SARS-CoV-2 RNA (GC/ reaction) | |
| US CDC N1 RT-qPCR | 97.7 | 0.99 | -3.37 | 36.3 | 9.50 |
| US CDC N2 RT-qPCR | 95.9 | 0.98 | -3.42 | 39.1 | 26.7 |
| CCDC N RT-qPCR | 100 | 0.98 | -3.31 | 36.9 | 16.4 |
| CCDC ORF1ab RT-qPCR | 98.0 | 0.99 | -3.37 | 37.4 | 11.6 |
| E_Sarbeco RT-qPCR | 94.0 | 0.99 | -3.48 | 39.8 | 48.1 |
| US CDC N1 RT-dPCR | - | - | - | - | 3.30 |
CCDC: China CDC.
