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. Author manuscript; available in PMC: 2022 Jul 7.
Published in final edited form as: J Am Soc Mass Spectrom. 2021 Apr 19;32(7):1601–1609. doi: 10.1021/jasms.0c00471

Figure 4. Compensation for adalimumab radical scavenging in TNFα footprinting.

Figure 4.

A. Flow injection real-time adenine dosimetry results for triplicate flow injections of TNFα at (black) 0V or (blue) 715V flash lamp drive voltage. Hydroxyl radicals generated at 715V drive voltage reduce the adenine absorbance at 265 nm by 39.7±1.95 mAU. B. Flow injection real-time adenine dosimetry results for triplicate flow injections of a 1:1 mixture of TNFα:adalimumab at (black) 0 V or (blue) 1000 V flash lamp drive voltage, giving an adenine ΔAbs265nm of 39.03±1.51 mAU.