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. Author manuscript; available in PMC: 2022 Jul 7.
Published in final edited form as: J Am Soc Mass Spectrom. 2021 Apr 19;32(7):1601–1609. doi: 10.1021/jasms.0c00471

Figure 5. FOX system HRPF of TNFα in the presence or absence of adalimumab.

Figure 5.

A. Histogram of FOX system HRPF peptide level results for TNFα alone (filled) or in the presence of adalimumab, compensated for scavenging (open) as reported by FoxWare Protein Footprinting software. (Inset) Peptide 66–82 results rescaled. Error bars represent one standard deviation from a triplicate measurement. Peptides that showed statistically significant protection upon adalimumab binding are marked with a red asterisk (p ≤ 0.05). B. Volcano plot of changes in TNFα FOX system HRPF results upon binding of adalimumab. Peptides that showed a statistically significant change that was at least a two-fold reduction in oxidation are colored red, while peptides that showed less than a two-fold reduction in oxidation are colored orange.