TABLE 1.
Samplea | Data before QC |
Data after processing |
Data after QC |
Data after alignment |
|||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Size (bp) | No. of sequence reads | Mean GC content (%) | Mean sequence length (bp) | No. of artificial duplicate read sequences | No. of known proteins predicted | No of RNA features predicted | Size (bp) | No. of sequence reads | Mean GC content (%) | Mean sequence length (bp) | No. of known proteins identified | No of RNA features identified | |
HT21 (HR) | 2,152,004,650.3 | 13,739,258.3 | 64 ± 10 | 154 ± 32 | 836,435 | 7,850,484.3 | 28,853 | 765,041,235.3 | 12,665,143.7 | 63 ± 9 | 155 ± 33 | 3,985,525.7 | 5,962.7 |
HT21 (DR) | 1,409,528,303.3 | 19,765,082 | 64 ± 10 | 155 ± 32 | 757,641.7 | 4,208,873.7 | 30,027 | 1,352,124,415 | 11,966,279.7 | 64 ± 9 | 156 ± 39 | 4,178,206.3 | 7,656.7 |
HT21 (BR) | 1,477,197,003 | 138,145,859.3 | 65 ± 9 | 155 ± 32 | 374,748 | 11,448,097.3 | 25,322.7 | 1,385,218,693 | 5,247,459 | 64 ± 9 | 156 ± 34 | 2,515,439.7 | 5,439.3 |
HR, healthy rhizosphere; DR, diseased rhizosphere; BR, bulk rhizosphere.