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. Author manuscript; available in PMC: 2022 Feb 3.
Published in final edited form as: Clin Sci (Lond). 2020 Jan 17;134(1):53–70. doi: 10.1042/CS20190794

Figure 1. Rictor silencing decreases the expression of Cdc42 and Rac1 and Cdc42 or Rac1 silencing inhibits PHT amino acid transport.

Figure 1.

(a, c) Representative western blot of Cdc42 and Rac1 expression in cell lysates of scramble siRNA and Raptor/Rictor silenced cells. Equal loading was performed. (b, d) Summary of the western blot data. Values are given as means + S.E.M.; n=4/each group. Means without a common letter differ significantly (P<0.05) by RMANOVA with Tukey–Kramer multiple comparisons post hoc test. (e-f) Silencing of Cdc42 or Rac1 inhibits System L amino acid uptake in PHT cells. (g-h) Silencing of Cdc42 or Rac1 inhibits System A amino acid uptake in PHT cells. Values are means + S.E.M.; n=6 /each group for System L and System A. *P<0.05 compared with control; unpaired Student's t test.