Figure 4.

Internal device operation. a) Cartoon of the NP detection assay chemistry at the analyte capture lines, which is based on NP binding to HRP-conjugated antibodies. Next, the NP conjugate was captured by the membrane bound capture antibody, followed by HRP turnover of the chromogenic substrate, DAB in the presence of H₂O₂. b) 3D model of the internal components of device. Sample introduction by swab into a swab port containing lysis buffer is followed by activation of the device, which causes puncture of aqueous reagent container and release of aqueous reagents into legs of the 2DPN. The 2DPN automates rehydration of dry reagents, splitting the sample into two channels (one for influenza A detection, the other for influenza B detection), and sequential delivery of subsequent assay reagents.