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. Author manuscript; available in PMC: 2022 Feb 3.
Published in final edited form as: Anal Chem. 2017 May 8;89(11):5776–5783. doi: 10.1021/acs.analchem.6b04801

Figure 5.

Figure 5

Examples of the extracted signal profiles for strong, weak, and negative results. The row-averaged pixel intensities in the blue channel of test strip images were used to generate plot profiles for each test strip (blue lines). Sixth-order polynomials (black lines) were fit to the background regions of each plot profile. Test lines were detected as thresholded (dotted black lines) excursions (red asterisks) of the background-subtracted plot profiles (red lines) beyond three standard deviations of the mean background-subtracted intensity (dotted black lines).