Figure 4.

Kiss1 mRNA in the arcuate nucleus (ARC) of fed to maintain (FM) and feed-restricted (FR) wethers. Confocal images (using a 10x objective) of Kiss1 cells from an FM (A) and FR (B) wether. Insets show high magnification confocal images (1 µm optical section, 63x objective) of representative Kiss1 cells in a FM (A inset) and a FR (B inset) wether. (C) Mean (±s.e.m.) number of Kiss1 cell bodies in the ARC were significantly lower (*P < 0.05) in FR wethers compared to FM wethers. (D) Mean (±s.e.m.) integrated density of Kiss1 mRNA per ARC cell was significantly lower (*P < 0.05) in FR wethers (n = 8) compared to FM wethers (n = 6). Scale bars, 100 µm. Scale bars for insets, 10 µm. For print clarity, Kiss1 mRNA has been pseudocolored green in these images. 3V, third cerebroventricle.