Figure 5.

(A and B) Representative photomicrographs (using a 10x objective) showing kisspeptin-immunoreactive (ir) cells and fibers in the middle arcuate nucleus (ARC) from a fed to maintain (FM; A) wether and a feed-restricted (FR; B) wether. Insets (using a 20x objective), arrows indicate kisspeptin-ir cell bodies. (C) Mean (±s.e.m.) number of kisspeptin-ir cell bodies per hemisection in the ARC was significantly lower (*P < 0.05) in FR wethers (n = 8) compared to FM wethers (n = 6). Scale bars, 200 µm. Scale bars for insets, 25 µm.