Figure 6.

NKB mRNA in the arcuate nucleus (ARC) of fed to maintain (FM) and feed-restricted (FR) wethers. Confocal images (using a 10x objective)) of NKB cells from an FM (A) and FR wether (B). Insets show high magnification confocal images (1 µm optical section, 63x objective) of representative NKB cells in a FM (A inset) and a FR (B inset) wether. (C) Mean (±s.e.m.) number of NKB cell bodies in the ARC were significantly fewer (*P < 0.05) in FR wethers (n = 8) compared to FM wethers (n = 6). (D) Mean integrated density of NKB mRNA per ARC cell was not significantly (NS) different between groups. Scale bars, 100 µm. Scale bars for insets, 10 µm. 3V, third cerebroventricle.