Figure 2:

Dietary prehabilitation results in restoration of SD stool microbiota composition after 3 days of prehabilitation. 16S rRNA sequencing was performed on stool microbiota for WD (n = 44), SD (n = 20), 3DP (n = 11), and 7DP (n = 10) mice prior to antibiotic exposure. (A.)The Shannon alpha diversity is reduced after dietary prehabilitation, and (B.) the beta diversity as measured by weighted unifrac demonstrates distinct clustering of the WD, SD, and prehabilitation (3DP and 7DP) stool microbiota. The composition of the stool microbitoa at the phyla level demonstrates similarities between SD, 7DP, and 3DP (C). (D) The abundance of Proteobacteria, Bacteroidetes (E), and Firmicutes (F) were compared across SD, WD, 3DP, and 7DP. * p< 0.05, ** p< 0.01, *** p< 0.001, **** p <0.0001