Fig. 2.

R72D07-GAL4 labels many neurons in the central brain, including a small group of mAL-resembling neurons: Expression of UAS-mCD8:GFP by R72D07-GAL4 reveals neurons in adult (A) VNC and (B) central brain. α-mCD8 (green) marks the R72D07-GAL4–targeted neurons. Neuropil marker α-NC82 (magenta) visualizes the brain and VNC. Among the neurons targeted by the R72D07-GAL4 is a small group of approximately three to five neurons per hemisphere with anatomical similarities to mAL. (C) Cell bodies of this group are located above ALs (yellow rectangle), like the mAL. (D) Processes of this group exhibit midline crossing (yellow arrow), and descend to the SOG, like the mAL. A and B are maximum projection through all the optical slices of the confocal stack. C and D are maximum projection of optical slices containing the cell bodies and processes of the mAL-resembling group, respectively. (Scale bars, 50 µm.)