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. 2022 Jan 26;119(5):e2117101119. doi: 10.1073/pnas.2117101119

Fig. 3.

Fig. 3.

Thermogenetic activation of mAL neurons increases lunges in intermale fights: Thermogenetic activation of mAL neurons at 30 °C increases (A) lunges but does not change (B) boxing, (C) UWE counts, or (D) UWE time, compared to the parental controls. At the control temperature of 22 °C, the phenotype of increased lunges is lost. For A–D, each circle represents lunges per 30 min, boxing events per 30 min, UWE counts per 30 min, and UWE time in seconds per 30 min, respectively. For A–D, Kruskal–Wallis test followed by post hoc Dunn’s was performed for both the temperatures (*P < 0.05, ***P <0.001, ns = not significant at P > 0.05). Genotypes and number of pairs used are indicated below each plot. (E) The mAL activation increases lunging without any effect on boxing or UWEs: The x axis represents the observation period, which is 1,800 s (or 30 min). Representative fights of (i) R43D01-GAL4/UAS-dTrpA1 at 22 °C (Left) and 30 °C (Right), (ii) R43D01-GAL4-only control at 22 °C (Left) and 30 °C (Right), and (iii) UAS-dTrpA1-only control at 22 °C (Left) and 30 °C (Right).