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. 2022 Jan 26;119(5):e2117101119. doi: 10.1073/pnas.2117101119

Fig. 4.

Fig. 4.

GABA transmission from activated mAL neurons is crucial for increased aggression: GAD1 RNAi in thermogenetically activated mAL fights attenuates the (A) increased lunging phenotype. For A–D, each circle represents lunges per 30 min, boxing per 30 min, UWE counts per 30 min, and UWE time in seconds per 30 min, respectively. For A–D, Kruskal–Wallis test followed by post hoc Dunn’s was performed for the control and experimental temperatures of 22 °C and 30 °C (*P < 0.05, **P < 0.01, ***P < 0.001, ns = not significant at P > 0.05), respectively. Genotypes and number of pairs used are shown below each plot. (E) GAD1 RNAi attenuates the increased lunging phenotype of mAL-activated fights: The x axis represents the observation period, which is 1,800 s (or 30 min). Representative fights of (i) R43D01-GAL4/UAS-dTrpA1 at 22 °C (Left) and 30 °C (Right), (ii) UAS-Dcr2; R43D01-GAL4/UAS-dTrpA1 at 22 °C (Left) and 30 °C (Right), (iii) UAS-Dcr2/UAS-GAD1 RNAi; R43D01-GAL4/UAS-dTrpA1 at 22 °C (Left) and 30 °C (Right), and (iv) UAS-Dcr2/UAS- no RNAi; R43D01-GAL4/UAS-dTrpA1 at 22 °C (Left) and 30 °C (Right).