Fig. 6.

Activation of a small group of mAL-resembling cells in the R72D07-GAL4 line is required for increased male aggression but not increased UWEs: Driving GAL80 expression with the R43D01-LexA driver attenuates the (A) increased lunging and (B) increased boxing of the thermogenetically activated R72D07-GAL4 fights without any effect on the (C) increased UWE counts or (D) increased UWE time. For A–D, each circle represents lunges per 30 min, boxing events per 30 min, UWE counts per 30 min, and UWE time in seconds per 30 min, respectively. For A–D, Kruskal–Wallis test followed by post hoc Dunn’s was performed for the control and experimental temperatures of 22 °C and 30 °C, respectively (*P < 0.05, **P < 0.01, ***P < 0.001, ns = not significant at P > 0.05). Genotypes and number of pairs used are shown below each plot. (E) R72D07-GAL4–labeled mAL-resembling neurons (green, α-mCD8) are GABA⁺ (red, α-GABA) and are denoted by white arrows. Overlap of signals in a single optical slice is shown for accurate representation. (Scale bars, 15 µm.)