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. 2022 Jan 31;23(1):76–88. doi: 10.1080/15384047.2021.2017222

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 1. — Identification of CAFs and EVs. The CAFs and NFs were successfully isolated from COAD tissues and adjacent normal tissues of patients undergoing colon cancer surgery. (a) Immunofluorescence was used to observe the morphology of CAFs and the expressions of surface markers vimentin, α-SMA, and FAP; (b) The purity of NFs and CAFs was detected using flow cytometry. The EVs were extracted. (c) The morphology of EVs was observed by TEM; (d) The size distribution of EVs was analyzed using NTA; (e) The expressions of CD9, CD63, and Calnexin were analyzed using WB.