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. 2022 Jan 31;23(1):76–88. doi: 10.1080/15384047.2021.2017222

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited, and is not altered, transformed, or built upon in any way.

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Figure 6. — Overexpression of FOXO1 reversed the effect of EVs on promoting angiogenesis. The FOXO1 was overexpressed in CAFs-EV-treated SW480/HCT116 cells. (a) The expression pattern of FOXO1 was detected using WB; (b) The proliferation of HUVECs was detected using CCK-8; (c) The migration was detected using Transwell; (d) The number of connections was measured to quantify the tube formation of HUVECs; (e) The VEGF content in HUVECs supernatant was detected using ELISA. Three cell tests were performed and the data were expressed as mean ± standard deviation. One-way ANOVA was used for comparison among groups. Tukey’s multiple comparisons test was used for the post hoc test. **P < .01.