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. 2022 Jan 31;29(1):440–453. doi: 10.1080/10717544.2022.2030428

Figure 4.

Figure 4.

MiR-141-3p knockdown suppresses the activation of HSCs by PTEN/AKT pathway. (A–G) LX2 cells were transfected with miR-141-3p inhibitor or NC inhibitor. (A) qRT-PCR analysis for the expression level of miR-141-3p in LX2 cells after transfection. (B, C) The proliferation ability analysis of LX2 cells using CCK-8 and EdU assays. (D, E) Flow cytometry for cell cycle and cell apoptosis in LX2 cells. (F, G) Western blot analysis for the protein levels of α-SMA, collagen I, PTEN, AKT, and p-AKT in LX2 cells. *p < .05, **p < .01, and ***p < .001.