Table 3.

CQA outcomes for optimization of cryoprotectants levels and FD procedure.

Parameter	Critical Quality Attribute (CQA)
	Cake appearance	Residual moisture (%)	Reconstitution Product	Reconstitution time (min)
Man: Suc (% w/v)	All samples prepared using unannealed FD protocol
4:1	Granular with large aggregates	11.34 ± 3.63	Significant precipitation	N/A
4:2	Granular with large aggregates	9.81 ± 2.85	Significant precipitation	N/A
6:2	Granular	10.28 ± 2.40	Some precipitation	N/A
FD protocol	All samples prepared with fixed Man: Suc at 6/2 %w/v
Unannealed	Granular with large aggregates	10.28 ± 2.40	Some precipitation	N/A
Conservative	Fine powder	4.47 ± 1.13a	Completely soluble	3.28 ± 0.49
Extensive	Fine powder	4.09 ± 0.91b	Completely soluble	2.52 ± 0.81

^{a, b} Residual moisture content was lower than that of unannealed FD samples, ρ < .05.