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. 2022 Feb 1;11(1):2029298. doi: 10.1080/2162402X.2022.2029298

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© 2022 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — MUC1-C→PBRM1/PBAF pathway induces IDO1, WARS and PTGES. (a and b). RNA-seq was performed in triplicates on DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days and then stimulated with 10 ng/ml IFN-γ for 24 hours. The datasets were analyzed by GSEA using the HALLMARK INTERFERON GAMMA RESPONSE gene signature comparing DOX-treated with vehicle-treated cells (a). Analysis of the indicated genes in vehicle- and DOX-treated cells showing significant differences in mRNA levels (b). (c). Expression of IDO1, WARS and PTGES in the TCGA-PRAD cohort was assessed in MUC1-high and MUC1-low groups using a Wilcoxon rank-sum test. (d). Lysates from DU-145/tet-CshRNA and DU-145/tet-MUC1shRNA treated with vehicle or DOX for 7 days were immunoblotted with antibodies against the indicated proteins. (e). Lysates from DU-145 expressing a CsgRNA or MUC1sgRNA#1 were immunoblotted with antibodies against the indicated proteins. (f). DU-145/tet-MUC1shRNA cells treated with vehicle or DOX for 7 days were stimulated with 10 ng/ml IFN-γ for 24 hours. Lysates were immunoblotted with antibodies against the indicated proteins. (g). Lysates from DU-145/CshRNA and DU-145/PBRM1shRNA cells were immunoblotted with antibodies against the indicated proteins. (h). Lysates from DU-145/CshRNA and DU-145/PBRM1shRNA cells stimulated with 10 ng/ml IFN-γ for 24 hours were immunoblotted with antibodies against the indicated proteins.