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. Author manuscript; available in PMC: 2022 Oct 1.
Published in final edited form as: Acta Neuropathol. 2021 Jun 21;142(4):629–642. doi: 10.1007/s00401-021-02330-2

Fig. 6.

Fig. 6

TMEM106B knockdown leads to increased insoluble TDP-43 proteins in an inducible cell system that expresses mislocalized TDP-43 protein. Sequential extractions of total protein lysate were performed at 48 and 72 hours after TMEM106B knockdown. a-f Representative immunoblot pictures of cell lysates at 48 hours after TMEM106B knockdown and associated densitometric analysis (n = 3 independent experiments) reveal a 2.5-fold increase in the RIPA-insoluble fraction of endogenous TDP-43 (a, c). g-l Representative immunoblot pictures of cell lysates at 72 hours after TMEM106B knockdown and associated densitometric analysis (n = 3 independent experiments) revealed a significant increase in the RIPA-insoluble fraction of both endogenous TDP-43 (g, i) and exogenously-expressed GFP-TDP-43 (g, j). * One-way ANOVA (TMEM106B knockdown vs. control), with Tukey posthoc pairwise comparison p < 0.05 corrected for multiple comparisons.