Table 1.
Cross-inhibitory activity of EK1 and EK1V1 against different subtypes of HIV-1 isolates (IC50 ± SD, μM)a.
| HIV-1 Env | Subtype | Cell fusion (IC50±SD, μM) | Pseudovirus infection (IC50±SD, μM) | ||
|---|---|---|---|---|---|
| EK1 | EK1V1 | EK1 | EK1V1 | ||
| 398-F1_F6_20 | A | > 5 | 0.17 ± 0.01 | > 12.5 | 1.2 ± 0.23 |
| TRO.11 | B | > 5 | 0.68 ± 0.17 | > 12.5 | 1.35 ± 0.28 |
| X2278_C2_B6 | B | > 5 | 0.05 ± 0.004 | > 12.5 | 0.88 ± 0.12 |
| JRFL | B | > 5 | 0.05 ± 0.001 | > 12.5 | 0.11 ± 0.02 |
| SF162 | B | > 5 | 0.05 ± 0.01 | > 12.5 | 0.1 ± 0.011 |
| CE703010217_B6 | C | > 5 | 0.04 ± 0.01 | > 12.5 | 0.2 ± 0.03 |
| HIV_25710-2.43 | C | > 5 | 0.17 ± 0.03 | > 12.5 | 0.71 ± 0.08 |
| CE1176_A3 | C | > 5 | 0.35 ± 0.1 | > 12.5 | 0.89 ± 0.02 |
| X1632-S2-B10 | G | > 5 | 0.04 ± 0.01 | > 12.5 | 0.85 ± 0.17 |
| 246_F3_C10_2 | A/C | > 5 | 0.05 ± 0.01 | > 12.5 | 1.03 ± 0.19 |
| CNE8 | A/E | > 5 | 0.49 ± 0.09 | > 12.5 | 1.23 ± 0.12 |
| CNE55 | A/E | > 5 | 0.13 ± 0.03 | > 12.5 | 3.02 ± 0.48 |
| CH119.10 | B/C | > 5 | 0.02 ± 0.004 | > 12.5 | 0.57 ± 0.04 |
| BJOX002000.03.2 | B/C | > 5 | 0.07 ± 0.01 | > 12.5 | 0.5 ± 0.03 |
| Mean | > 5 | 0.17 | > 12.5 | 0.9 | |
a
The assay was performed in triplicate and repeated three times, and data are expressed as means ± SD.