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. 2022 Jan 24;18(1):e1009718. doi: 10.1371/journal.ppat.1009718

Fig 3. Salmonella induces NAIP/NLRC4- and NLRP3-dependent inflammasome activation in human macrophages.

Fig 3

(A, C, E) WT, NAIP-/-, or NLRC4-/- THP-1 monocyte-derived macrophages were primed with 100 ng/mL Pam3CSK4 for 16 hours. One hour prior to infection, cells were treated with 1 μM MCC950, a chemical inhibitor of the NLRP3 inflammasome, or DMSO as a control. Cells were then infected with PBS (Mock), WT S. Typhimurium, or ΔsipB S. Typhimurium at an MOI = 20 for 6 hours. (B, D) As a control, cells were primed with 500 ng/mL LPS for 4 hours and treated with 10 μM nigericin for 6 hours. (A–D) Release of IL-1β into the supernatant was measured by ELISA. (E) Immunoblot analysis was performed on supernatants (sup) for mature IL-1β and on lysates for pro–IL-1β and β-actin as a loading control. ns–not significant, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 by Tukey’s multiple comparisons test (A, C) or by Šídák’s multiple comparisons test (B, D). Error bars represent the standard deviation of triplicate wells from one experiment. Data shown are representative of at least three independent experiments.