FIGURE 1.

Quantification of HoxB8-conditional progenitor engraftment in unconditioned mice. (A) CD45.1 mice received transplantation of 1 × 10⁸ progenitors of one of the three indicated HoxB8-conditional progenitor cell lines that were independently established as described in the Methods. The fraction of all CD45⁺ bone marrow cells that were derived from transplanted HoxB8-conditional progenitors (CD45.2⁺) in bone marrow of recipient mice at 5 days after transplantation. HoxB8-conditional progenitor line 1, line 2, and line 3 are indicated here as P1, P2, and P3, respectively. Data were analyzed using one-way ANOVA. *p < 0.05. (B) CD45.1 mice received transplantation of 1 × 10⁸ HoxB8-conditional progenitors (line 1) to perform a time course of both the fraction (blue, left y-axis) and absolute number (red, right y-axis) of donor-derived cells in the bone marrow. (C) The donor-derived (CD45.2⁺) fraction of all mature Ly6G^high neutrophils in the bone marrow CD45.1 mice that received varying doses of HoxB8-conditional progenitors, measured at 7 days post-transplant. (D) Evaluation of the maximum fraction of donor-derived (CD45.2⁺) neutrophils in the peripheral blood of CD45.1 mice that received transplant of 3 × 10⁷ cells of either the parental HoxB8-conditional progenitor cell line 1 or one of the single-cell clones derived from that cell line. Data were analyzed using one-way ANOVA. *p < 0.05, compared to parental line 1.