FIGURE 3.

HoxB8-conditional progenitor engraftment and in vivo differentiation. (A) Five days after CD45.1 mice received a 1:1 mix of wild-type and the indicated gene-deficient HoxB8-conditional progenitors, the relative frequency of wild-type and gene knockout cells among all donor-derived cells in the bone marrow was measured by flow cytometry. Data are presented as the relative frequency of each gene knockout type relative to its wild-type counter part, and normalized to the wild-type control (set to equal 1.0). Data were analyzed using one-way ANOVA. *p < 0.05, compared to wild-type. (B) Example flow cytometry dotplots showing analyses of whole bone marrow from CD45.1 mice that received transplant of CD45.2⁺ HoxB8-conditional progenitors. Samples were labeled to determine expression of CD45.1, CD45.2, cKit and Ly6G. Gating for analyzing only donor-derived cells (top) and the subpopulations of donor-derived cells (bottom) in various states of maturity from progenitors/immature neutrophils (cKit⁺Ly6G^−/low) towards terminal neutrophils (cKit⁻Ly6G^high). (C) Characterization and quantification of the in vivo differentiation of engrafted HoxB8-conditional progenitors at day 4 and day 7 after transplantation.