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. 2022 Jan 24;16(1):e0010151. doi: 10.1371/journal.pntd.0010151

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© 2022 Mekonnen et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Relative mRNA expression levels of Sh-tsp-2 (A), Sh-tsp-4 (B), Sh-tsp-5 (C), Sh-tsp-6 (D), Sh-tsp-18 (E) and Sh-tsp-23 (F) were analyzed by qPCR. Relative mRNA expression levels were normalised to a housekeeping gene (α-tubulin), calculated using the 2^−ΔΔCt method using the egg stage as a reference group.