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. 2022 Jan 24;18(1):e1010255. doi: 10.1371/journal.ppat.1010255

Fig 3. IL-6 is required for the induction of adaptive immune responses by the mRNA-LNP platform.

Fig 3

A. The indicated mice were injected intradermally with 2.5 μg of non-coding polycytosine mRNA-LNP and the skin samples tested for IL-6 with Luminex 24 hours later. B. and C. WT and IL-6 knockout mice were intradermally injected with 10 μg of mRNA-LNP coding for PR8 HA or PBS. Seven days later the Tfh cells were characterized in skin draining lymph nodes using flow cytometry. D. as in B and C, except the lymph nodes were harvested 14 days post injection and the GC B cell responses determined by flow cytometer. Each dot represents a separate animal. E. Serum samples at day 14 post injection were assessed for anti-HA IgG levels using ELISA. F. The indicated animals were immunized with 10 μg of mRNA-LNP coding for PR8 HA or injected with PBS. Fourteen days later the animals were challenged with 5,000 TCDI PR8 influenza virus and the weight drop monitored as presented. Data from two independent experiments pooled, 5–9 mice/group.