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. 2022 Jan 24;11:e74387. doi: 10.7554/eLife.74387

Figure 7. Interactions with the PSD, but not with the membrane or actin cytoskeleton are required for positioning of the EZ.

Figure 7.

(A) Example images of dendrites expressing Homer1c-ALFA and endocytic proteins fused to GFP co-expressed with control or mirShank-mCherry construct. Scale bar: 2 µm. (B) Fraction of PSDs associated with an EZ after Shank-KD relative to control plotted as mean ± SEM. GFP-CLCa (N = 8, p < 0.001), β2-adaptin (N = 10, p < 0.01), Eps15 (N = 12, p < 0.05), Itsn1L (N = 10, p < 0.05), HIP1R (N = 11 p > 0.05), Dyn2 (N = 12, p > 0.05), CPG2 (N = 13, p > 0.05). (C) Illustration of possible mechanisms that could maintain the EZ adjacent to the PSD. (D) Example images of dendrites co-expressing Homer1c-ALFA and GFP-CLCa in dendrites treated with LatB, CK666, or Jasp. Scale bar: 2 µm. (E) Example images of dendrites expressing control constructs, CLCb-EED/QQN (left panel) and AP2m2-P1 (middle panel), or Itsn1 KD construct (mirItsn1; right panel). Scale bar: 2 µm. (F) Fraction of EZ-associated PSDs relative to control, plotted as mean ± SEM. Jasp (N = 4), LatB (N = 8), CK666 (N = 12), CLCb-EED/QQN (N = 7), AP2m2-P1 (N = 5), mirItsn1 (N = 11), shCPG2 (N = 8).

Figure 7—source data 1. Excel sheet with numerical data represented as plots Figure 7D and F.