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. 2020 Dec 31;17(1):1–18. doi: 10.1080/15592294.2020.1864170

Figure 7.

Figure 7.

Impact of the N-terminal acetylation on H4R3me methylation by PRMT1 and PRMT5. (a) Single-point radioactive methylation assay performed with 0.05 µM PRMT1, 15 µM [14C]SAM, and 15 µM of peptide substrate at 30°C for 10 min. (b) Single-point radioactive methylation assay performed with 0.25 µM PRMT5, 15 µM [14C]SAM, and 15 µM of peptide substrate at 30°C for 10 min. (c) PRMT1 kinetics with substrate NH2-H4(1–20)R3me. Reactions were held for 15 min at 30°C with 0.05 µM PRMT1 and 15 µM [14C]SAM. All error bars represent standard deviation.