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Assessment of bladder cancer cell viability and recovery following a single freeze event.
SCaBER (A) cells were subjected to a 5 minute freeze at −10, −15, −20, and −25°C and survival was assessed over seven days post-treatment. Data suggest that complete cell death with no recovery is attained following exposure to −25°C whereas −15°C and −20°C exposure results in a substantial level of cell death followed by recovery in culture.
