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. Author manuscript; available in PMC: 2022 Feb 3.
Published in final edited form as: Clin Res (Milpitas). 2020 Feb 7;6(1):10.16966/2469-6714.154. doi: 10.16966/2469-6714.154

Figure 2:

Figure 2:

Impact of repeat freeze on bladder cancer cell viability and recovery. SCaBER and UMUC3 cells were subjected to a double 5 minute freeze (5/10/5) at −10, −15, −20, and −25°C and survival was assessed over seven days post-treatment. Data suggest that a double freeze at −20°C results in complete bladder cancer cell death with no recovery. Double freeze to −15°C resulted in a significant decrease in cell survival. However a low level of recovery was noted over the 7 day assessment interval.