Table 2.

Primer and Probe Sets for Genomic Regions Targeted by the SARS-CoV-2 Nucleocapsid LDT and SARS-CoV-2 RdRp LDT

Target	Name	Sequence	Nucleotide position
N	SARS-CoV-2 N1-F	5′-GACCCCAAAATCAGCGAAAT-3′	28,287–28,306
	SARS-CoV-2 N1-R	5′-TCTGGTTACTGCCAGTTGAATCTG-3′	28,335–28,358
	SARS-CoV-2 N1-Probe	5′-FAM-ACCCCGCATTACGTTTGGTGGACC-BHQ1-3′	28,309–28,332
	SARS-CoV-2 N2-F	5′-TTACAAACATTGGCCGCAAA-3′	29,164–29,183
	SARS-CoV-2 N2-R	5′-GCGCGACATTCCGAAGAA-3′	29,213–29,230
	SARS-CoV-2 N2-Probe	5′-ABY-ACAATTTGCCCCCAGCGCTTCAG-QSY-3′	29,188–29,210
RdRp	nCoV_IP2-F	5′-ATGAGCTTAGTCCTGTTG-3′	12,690–12,707
	nCoV_IP2-R	5′-CTCCCTTTGTTGTGTTGT-3′	12,780–12,797
	nCoV_IP2-Probe	5′-HEX-AGATGTCTT/ZEN/GTGCTGCCGGTA-IABkFQ-3′	12,717–12,737
	nCoV_IP4-F	5′-GGTAACTGGTATGATTTCG-3′	14,080–14,098
	nCoV_IP4-R	5′-CTGGTCAAGGTTAATATAGG-3′	14,167–14,186
	nCoV_IP4-Probe	5′-HEX-TCATACAAA/ZEN/CCACGCCAGG-IABkFQ-3′	14,105–14,123
RNase P	Human RP-F	5′-AGATTTGGACCTGCGAGCG-3′	g.90,872,001_90,872,019
	Human RP-R-S3	5′-ATAGCAACAACTGAATAGCCAAGG-3′	c.94_117
	Human RP-Probe	5′-JUN-TTCTGACCTGAAGGCTCTGCGCG-QSY-3′	g.90,872,022_90,872,044
PPIA	PPIA-F	5′-CATCCTAAAGCATACGGGTCC-3′	g.44,799,776_44,799,796
	PPIA-R	5′-TCTTTCACTTTGCCAAACACC-3′	g.44,801,305_44,801,325
	PPIA-Probe	5′-Cy5-TGCTTGCCATCCAACCACTCAGTC-IAbRqSp-3′	c.398_421

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) targets accession number NC_045512.2; RNaseP accession number NM_006413.5; peptidylprolyl isomerase A (PPIA) accession number NM_021130 (all available from https://www.ncbi.nlm.nih.gov/nuccore). Reference Genome Assembly: GRCh38/hg38 (available from https://www.ncbi.nlm.nih.gov/assembly).

LDT, laboratory-developed test; RdRp, RNA-dependent RNA polymerase.